Research oligonucleotides

Supporting research

Oligos are essential tools in nucleic acids research, supporting a wide range of molecular, genetic and synthetic biology applications.

From standard DNA and RNA sequences to complex modified oligos, ATDBio provides synthesis, purification and analytical support tailored to your project requirements.

ATDBio supports oligos used across a wide range of nucleic acid research, including:

Scale, Purification & Analysis

Custom oligos are available from 0.2 µmol to 1 mmol scale, supporting early-stage research through to larger-scale project requirements.

All oligos are checked by LC-MS as standard to confirm identity and purity. Tailored analytical support is also available for more complex oligos, modified sequences or project-specific requirements.

Oligo Formats

Custom oligos in a range of formats for complex research applications.

• Single-stranded DNA and RNA
• Duplex oligos
• Long and highly modified oligos
• Aptamers
• NGS adapters
• Branched oligo structures

Gene Regulation Tools & RNA

Modified oligos for RNA biology, gene regulation and functional genomics.

• RNA interference applications
• siRNA
• miRNA
• Antisense oligos
• Aptamers
• sgRNA
• Modified RNA oligos

Reactive & Functional Chemistries

Reactive chemistries to support click chemistry, conjugation and downstream modification.

• Azide / alkyne click chemistry (Cu catalysed)
• DBCO / BCN click chemistry (Cu-free)
• TCO / tetrazine chemistry
• Amino, thiol and maleimide modifiers
• Photoreactive and photocleavable modifications

Detection Labels & Functional Mods

Labels and functional modifications for detection, imaging, tracking and assay development.

• Fluorescent dyes
• Quenchers, including internal quenchers
• Biotin, desthiobiotin, digoxigenin and other affinity tags
• Redox labels
• Probe-related modifications
• Spacers, PEG linkers and functional handles

Modified Bases

Modified bases for studying nucleic acid biology, epigenetic regulation and RNA/DNA modification pathways.

• Epigenetic bases: N6-Methyladenosine, 5-Hydroxymethylcytosine and 5-Formylcytosine
• Modified RNA bases: Pseudouridine and N1-Methyladenosine
• Fluorescent base modifications: including pyrrolo-dC
• Damage-related bases: including oxidative damage base modifications
• Universal and degenerate base options

Sugar, Backbone & Oligo Chemistries

Specialist chemistries to support stability, binding affinity, nuclease resistance and oligo performance.

• Sugar modifications: 2′-O-Methyl, 2′-Fluoro, 2′-MOE, LNA and cEt
• Specialist oligo chemistries: TNA, HNA, GNA, iso-GNA, FNA and 2′-F-ANA
• Backbone chemistries: PS, MeP, MsPA and other backbone chemistries
• Terminal chemistries: 5′-vinyl phosphonates and GalNAc analogues
• Hydrophobic modifications: lipids, cholesterol and 2′-O-C16/C22