Phosphate modification

In convential solid-phase oligonucleotide synthesis, oligos are synthesized with a hydroxyl (OH) group at both the 5′- and the 3′-end. For some applications it is necessary to have a phosphate group at the 5' or 3′-end. (Figure 1).

Structures of oligonucleotides containing 5'- and 3'-phosphate modifications

Figure 1 | Structures of oligonucleotides containing 5′- and 3′-phosphate modifications

Applications in which terminal phosphate groups are used include enzymatic ligation of oligonucleotides and blocking of PCR. For further information see The 5′-phosphate monomer and gene synthesis and The 3′-phosphate group as a PCR blocker.

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