Call us: +44 (0)23 80596778 / Request a quote online
In convential solid-phase oligonucleotide synthesis, oligos are synthesized with a hydroxyl (OH) group at both the 5′- and the 3′-end. For some applications it is necessary to have a phosphate group at the 5' or 3′-end. (Figure 1).
Applications in which terminal phosphate groups are used include enzymatic ligation of oligonucleotides and blocking of PCR. For further information see The 5′-phosphate monomer and gene synthesis and The 3′-phosphate group as a PCR blocker.
We wrote the book. Read our Nucleic Acids Book, an exclusive online guide to the chemistry and biology of nucleic acids.
Tell us about your problem, and we'll use all of our knowledge of nucleic acids chemistry to help you solve it.