Produced by OmniGraffle 7.18.5\n2015-08-10 14:14:00 +0000
Canvas 1
Layer 1
HO
HO
O
DNA fragment
adaptor
‘sticky’ end
HO
PO
2-
4
OH
O P
2-
4
‘blunt’ end
+
O
-
O
P
O
O
H
O
-
O
-
O
P
O
5’
3’
3’
5’
fragment ends are attracted
to one another by H bonding
3’
5’
5’
3’
3’
5’
ligation of two fragments can occur
modified adaptor with 5’-OH terminus
5’
3’
3’
5’
dimerization cannot occur
Ligase
Ligase
Ligase
genomic DNA
double-stranded DNA fragments
sonication or
digestive enzyme